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Evidence that an Unconventional Actin Can Provide Essential F-Actin Function and that a Surveillance System Monitors F-Actin Integrity in Chlamydomonas

Masayuki Onishi and Frederick R. Cross Genetics. 2016 Mar;202(3):977-96. doi: 10.1534/genetics.115.184663. Epub 2015 Dec 29. Actin is one of the most conserved eukaryotic proteins. It is thought to have multiple essential cellular roles and to function primarily or exclusively as filaments (“F-actin”). Chlamydomonas has been an enigma, because a null mutation (ida5-1) in its single … Continue reading Evidence that an Unconventional Actin Can Provide Essential F-Actin Function and that a Surveillance System Monitors F-Actin Integrity in Chlamydomonas



High-Throughput Genotyping of Green Algal Mutants Reveals Random Distribution of Mutagenic Insertion Sites and Endonucleolytic Cleavage of Transforming DNA

Zhang R, Patena W, Armbruster U, Gang SS, Blum SR, Jonikas MC Plant Cell. 2014 Apr 4;26(4):1398-1409 A high-throughput genetic screening platform in a single-celled photosynthetic eukaryote would be a transformative addition to the plant biology toolbox. Here, we present ChlaMmeSeq (Chlamydomonas MmeI-based insertion site Sequencing), a tool for simultaneous mapping of tens of thousands … Continue reading High-Throughput Genotyping of Green Algal Mutants Reveals Random Distribution of Mutagenic Insertion Sites and Endonucleolytic Cleavage of Transforming DNA