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Photoactivated adenylyl cyclases as optogenetic modulators of neuronal activity

Costa WS, Liewald J, Gottschalk A Methods Mol Biol. New York, NY: Springer New York; 2014;1148(Chapter 11):161–75 In recent years, optogenetic methods became invaluable tools, particularly in neurobiological research. Most prominently, optogenetic methods utilize microbial rhodopsins to elicit neuronal de- or hyperpolarization. However, other optogenetic tools have emerged that allow influencing neuronalfunction by different approaches. … Continue reading Photoactivated adenylyl cyclases as optogenetic modulators of neuronal activity



CASP, the alternatively spliced product of the gene encoding the CCAAT-displacement protein transcription factor, is a Golgi membrane protein related to giantin

Gillingham AK, Pfeifer AC, Munro S Mol Biol Cell. 2002 Nov;13(11):3761–74 Large coiled-coil proteins are being found in increasing numbers on the membranes of the Golgi apparatus and have been proposed to function in tethering of transport vesicles and in the organization of the Golgi stack. Members of one class of Golgi coiled-coil protein, comprising … Continue reading CASP, the alternatively spliced product of the gene encoding the CCAAT-displacement protein transcription factor, is a Golgi membrane protein related to giantin



Enhanced yeast one-hybrid assays for high-throughput gene-centered regulatory network mapping

Reece-Hoyes JS, Diallo A, Lajoie B, Kent A, Shrestha S, Kadreppa S, Pesyna C, Dekker J, Myers CL, Walhout AJ Nat Methods. 2011 Oct 30;8(12):1059-64 Yeast/2-Hybrid A major challenge in systems biology is to understand the gene regulatory networks that drive development, physiology and pathology. Interactions between transcription factors and regulatory genomic regions provide the … Continue reading Enhanced yeast one-hybrid assays for high-throughput gene-centered regulatory network mapping