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Dual mutations reveal interactions between components of oxidative phosphorylation in Kluyveromyces lactis

Clark-Walker GD, Chen XJ Genetics. 2001 Nov;159(3):929–38 Loss of mtDNA or mitochondrial protein synthesis cannot be tolerated by wild-type Kluyveromyces lactis. The mitochondrial function responsible for rho(0)-lethality has been identified by disruption of nuclear genes encoding electron transport and F(0)-ATP synthase components of oxidativephosphorylation. Sporulation of diploid strains heterozygous for disruptions in genes for the … Continue reading Dual mutations reveal interactions between components of oxidative phosphorylation in Kluyveromyces lactis



The F1-ATPase inhibitor Inh1 (IF1) affects suppression of mtDNA loss-lethality in Kluyveromyces lactis

Clark-Walker GD FEMS Yeast Res. 2007 Aug;7(5):665–74 Loss of mtDNA by the petite-negative yeast Kluyveromyces lactis is lethal (rho(o)-lethality). However, mutations in the alpha, beta and gamma subunits of F(1)-ATPase can suppress lethality by increasing intramitochondrial hydrolysis of ATP. Increased hydrolysis of ATP can also occur on inactivation of Inh1, the natural inhibitor of F(1)-ATPase. … Continue reading The F1-ATPase inhibitor Inh1 (IF1) affects suppression of mtDNA loss-lethality in Kluyveromyces lactis