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Pervasive function and evidence for selection across standing genetic variation in S. cerevisiae

Christopher M. Jakobson, Richard She & Daniel F. Jarosz Nature Communicationsvolume 10, Article number: 1222 (2019) Quantitative genetics aims to map genotype to phenotype, often with the goal of understanding how organisms evolved. However, it remains unclear whether the genetic variants identified are exemplary of evolution. Here we analyzed progeny of two wild Saccharomyces cerevisiae … Continue reading Pervasive function and evidence for selection across standing genetic variation in S. cerevisiae



PIXL EFFICIENCY REPORT: AGAR-AGAR

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Genetics of single-cell protein abundance variation in large yeast populations

Frank W. Albert, Sebastian Treusch, Arthur H. Shockley, Joshua S. Bloom & Leonid Kruglyak Nature volume 506, pages 494–497 (27 February 2014) Variation among individuals arises in part from differences in DNA sequences, but the genetic basis for variation in most traits, including common diseases, remains only partly understood. Many DNA variants influence phenotypes by … Continue reading Genetics of single-cell protein abundance variation in large yeast populations



Incomplete proteasomal degradation of green fluorescent proteins in the context of tandem fluorescent protein timers

Anton Khmelinskii, Matthias Meurer, Chi-Ting Ho, Birgit Besenbeck, Julia Füller, Marius K. Lemberg, Bernd Bukau, Axel Mogk, and Michael Knop Mol Biol Cell. 2016 Jan 15;27(2):360-70. doi: 10.1091/mbc.E15-07-0525. Epub 2015 Nov 25. Tandem fluorescent protein timers (tFTs) report on protein age through time-dependent change in color, which can be exploited to study protein turnover and … Continue reading Incomplete proteasomal degradation of green fluorescent proteins in the context of tandem fluorescent protein timers



Genetic variation and expression changes associated with molybdate resistance from a glutathione producing wine strain of Saccharomyces cerevisiae

Francesco Mezzetti, Justin C. Fay, Paolo Giudici, Luciana De Vero July 6, 2017 https://doi.org/10.1371/journal.pone.0180814 Glutathione (GSH) production during wine fermentation is a desirable trait as it can limit must and wine oxidation and protect various aromatic compounds. UMCC 2581 is a Saccharomyces cerevisiae wine strain with enhanced GSH content at the end of wine fermentation. … Continue reading Genetic variation and expression changes associated with molybdate resistance from a glutathione producing wine strain of Saccharomyces cerevisiae



Variation of the meiotic recombination landscape and properties over a broad evolutionary distance in yeasts

Christian Brion, Sylvain Legrand, Jackson Peter, Claudia Caradec, David Pflieger, Jing Hou, Anne Friedrich, Bertrand Llorente, Joseph Schacherer PLoS Genet. 2017 Aug 1;13(8):e1006917. doi: 10.1371/journal.pgen.1006917. eCollection 2017 Aug. Meiotic recombination is a major factor of genome evolution, deeply characterized in only a few model species, notably the yeast Saccharomyces cerevisiae. Consequently, little is known about … Continue reading Variation of the meiotic recombination landscape and properties over a broad evolutionary distance in yeasts



The regulatable MAL32 promoter in Saccharomyces cerevisiae: characteristics and tools to facilitate its use

Matthias Meurer, Veronika Chevyreva, Bram Cerulus, Michael Knop PLoS Genet. 2017 Aug 1;13(8):e1006917. doi: 10.1371/journal.pgen.1006917. eCollection 2017 Aug. Here we describe a set of tools to facilitate the use of maltose and the MAL32 promoter for regulated gene expression in yeast, alone or in combination with the GAL1 promoter. Using fluorescent protein reporters we find … Continue reading The regulatable MAL32 promoter in Saccharomyces cerevisiae: characteristics and tools to facilitate its use



Decoding the chromatin proteome of a single genomic locus by DNA sequencing

Tessy Korthout, Deepani W. Poramba-Liyanage, Ila van Kruijsbergen, Kitty F. Verzijlbergen, Frank P. A. van Gemert, Tibor van Welsem, Fred van Leeuwen. PLoS Biol. 2018 Jul; 16(7): e2005542. Transcription, replication, and repair involve interactions of specific genomic loci with many different proteins. How these interactions are orchestrated at any given location and under changing cellular … Continue reading Decoding the chromatin proteome of a single genomic locus by DNA sequencing



The Hidden Complexity of Mendelian Traits across Natural Yeast Populations

Jing Hou, Anastasie Sigwalt, David Pflieger, Jackson Peter, Jacky de Montigny, Maitreya Dunham, Joseph Schacherer Cell Rep. 2016 Jul 26;16(4):1106-1114. doi: 10.1016/j.celrep.2016.06.048. Epub 2016 Jul 7. Mendelian traits are considered to be at the lower end of the complexity spectrum of heritable phenotypes. However, more than a century after the rediscovery of Mendel’s law, the … Continue reading The Hidden Complexity of Mendelian Traits across Natural Yeast Populations