How to digest tetrads with Zymolyase
- Pick a sporulated culture with a toothpick
- Add spores to 50 µl dH2O
- Add 1ul of 5 U/µl Zymolyase (E1004B; Zymo Research) into the culture
- Leave at room temperature for 5 minutes. Check the efficiency of Zymolyase under the microscope.
- Repeat step 4 until the asci are digested to the correct level.
- Add 150 µl dH2O
- Spread a small amount of digested cells on plates