349 Citations Found
https://ir.library.osaka-u.ac.jp/repo/ouka/all/1506/22691_論文.pdf
村戸, 康人 Vertebrate head is a complex structure that consists of various kinds of tissues and organs. Among them, cranial sensory organs and ganglions are derived from special cell groups, called placodes. Based on the facts that they differentiated into multiple cell types and that are only found in vertebrate lineages, placodes are interesting subjects of studies from the perspectives of both ontogeny and phylogeny. Lines of studies have suggested that all placodes are derived from the pre-placodal ectoderm (PPE) that is boundary between future epidermis region and future neural region. A Xenopus gene Xhairy2 is, to our knowledge, the earliest gene that shows clear expression in PPE. The present study aimed at elucidating a function(s) of Xhairy2 in the formation of placodes by examining formation of cranial sensory organs. Morphological analyses of Xhairy2 morphants revealed that ocular lens was severely malformed though retinal structure looked normal. Vertebrate eyes are divided into retina and lens in terms of their origins: retina is formed from a part of diencephalons, while lens is developed from placode. Detailed marker gene analyses of eye in Xhairy2 morphants showed that the expression of all lens marker genes tested was reduced or abolished, while that of retinal marker genes was not affected. These results indicated that Xhairy2 was specifically required for lens development. Ectopic expression of p27xic1 by means of Xhairy2 knockdown was identified as a first trigger of lens malformation. First, apoptosis was induced in Xhairy2 morphants, which is a known marker of ectopic expression of p27xic1. Second, overexpression of p27xic1 mimicked phenotypes of Xhairy2 morphants. Finally, the phenotypes of Xhairy2 knockdown were partially rescued by simultaneous knockdown of p27xic1. Since p27xic1 is a cell-cycle inhibitor, it was assumed that the number of lens precursor cells was drastically reduced. To test this assumption, cell proliferation within regions of lens induction was examined by means of BrdU incorporation analyses in Xhairy2 morphants. However, no significant changes were observed at the onset of lens induction. Furthermore, lens induction occurred normally in the embryos treated with drugs that directly inhibit cell cycle. Collectively, the results suggested that Xhairy2 repressed the differentiation inducing activity, another activity of p27xic1. Placodes start differentiation long after the first fate decision. Therefore, it is quite important how they maintain stem-cell-like undifferentiated states. As Xhairy2 did not seem to be a master regulator of cascade of lens transcription factors and was shown to be involved in inhibition of differentiation via repression of p27xic1 expression, it was suggested that the undifferentiated states was actively maintained by early PPE expression of Xhairy2. The cascade of differentiation beginning with a signal input would normally work only when this kind of fundamental cell state, competence, exists.https://www.cell.com/developmental-cell/pdf/S1534-5807(13)00730-2.pdf
Ioanna Antoniades, Panayiota Stylianou, and Paris A. Skourides Developmental Cell 28, 70–80, January 13, 2014 Cilia have been associated with diverse developmental and physiological processes, and defects in cilia underlie a number of genetic conditions. Several lines of evidence support a critical role of the actin cytoskeleton in ciliogenesis and ciliary function. Here, we show that well-characterized focal adhesion (FA) proteins, including FAK, Paxillin, and Vinculin, associate with the basal bodies of multiciliated cells and form complexes (CAs) that interact with the actin cytoskeleton. FAK downregulation leads to ciliogenesis defects similar to those observed when the actin cytoskeleton is disrupted, including defects in basal body migration, docking, and spacing, suggesting that CAs link basal bodies to the actin cytoskeleton. The important role of FA proteins in ciliogenesis leads us to propose that evolutionarily FA proteins, many of which are found in primitive flagellated unicellular eukaryotes, may have originally evolved to perform functions at flagella and were later co-opted for use in cell adhesion.