What plates can I use with PhenoBooth?
The PhenoBooth accepts Singer PlusPlates and any SBS-format rectangular plates. Want to colony count on 90mm Petri dishes? We’ve got an adaptor for that too.
How many plates does PhenoBooth take?
PhenoBooth takes one plate at a time to ensure that every plate received identical lighting and exposure setting, which is necessary to maximize data quality and save time on downstream analysis.
How fast is PhenoBooth?
It takes PhenoBooth only 5 seconds to image one plate. This means that you could image up to 720 plates in an hour. It is that fast!
Does it come with a stacker?
No, it doesn’t. Because PhenoBooth is so fast, you don’t need to waste time loading plates onto a stacker.
What type of lighting is available in PhenoBooth?
PhenoBooth offers two types of lighting options: transmitted (back) light to maximize contrast between colonies and background, and incident (top) light to capture colour. The PhenoBooth is available in two different LEDs set-ups. The standard PhenoBooth includes UV, blue and white light. The PhenoBooth Cyan option includes cyan, blue and white light.
What is the Colony Picking Platform?
The Colony Picking Platform is made up of the PhenoBooth, the ROTOR HDA and The Stinger. Image randomly arrayed colonies using the PhenoBooth, then filter and select colonies of interest using the Colony Picking software package. The colony coordinates can be exported and loaded into the ROTOR HDA screening robot and picked into an array by The Stinger single colony picker head.
What exactly does the software do?
PhenoBooth’s software is a powerful solution with an intuitive UI for colony measurements and analysis. It consists of 6 fully integrated steps, including Image Acquisition, Pre-processing, Detection, Filtering, Analysis and Visualisation. Fully automate your favourite workflow.
What colony features does the PhenoBooth measure?
The PhenoBooth measures most phenotypes, including size, colour, circularity and brightness. If you want to make a measurement that doesn’t currently exist, just contact us and we’ll write the plugin!
Can the PhenoBooth detect sub-cellular or low levels of fluorescent expression?
The PhenoBooth is not a microscope! It is a macroscopic CCD imaging workstation. As such it will not detect sub-cellular or low levels of expression. The in-house strains used to successfully test PhenoBooth’s fluorescence detection have a fluorophore molarity of 1.84E-30 nM per cell. As such we cannot guarantee fluorescence detection below this value.
Can I perform data analysis as well?
Absolutely! The PhenoBooth is your one-stop solution for SGA analysis. It measures and normalises colony features, compares measurements on experimental and control plates, identifies and presents “hits” on a heat map or a chart. In addition, there will be extra plugins and packages available to customise workflow that deviate from standard SGA analysis.
How many software Licenses do I get?
You get three 1-year user licenses as standard! Don’t worry, you’ll still be able to use the PhenoBooth software with an expired license, but will miss out on some additional benefits. Additional licenses can be purchased at any time.
Help! I need PhenoBooth to do THIS for me!
SI constantly makes new software plugins for the PhenoBooth to fulfil different analysis needs. Please contact us if you have a good idea for what PhenoBooth should do next. We’d love to hear from you!