What is it?
The PhenoBooth+ Colony Counter is a fast, super-easy to use, closed-chamber Colony Counter for imaging and analysis of colonies on agar. It uses a scientific-grade camera and produces 23-megapixel plate images. It includes five lighting channels to capture high-resolution colony images from Petri dishes and rectangular plates.
How does it work?
Insert a Petri dish with visible bacteria, yeast or other microorganisms into the input tray; select your preferred wavelength; autofocus and capture your plates in seconds. Filter for properties of interest just once; hit ‘Batch Analyse’ and walk away as PhenoBooth+ automates the tedious analysis across all of your plates with a single click.
Advanced Detection
Colonies can be filtered by size, colour, circularity and brightness. Applications include Colony Counting, Growth Curves, and Fluorescence (BFP/GFP/YFP/mCherry*) Detection.
*The PhenoBooth+ is not a microscope! It is a macroscopic CCD imaging workstation. As such it will not detect sub-cellular or low levels of expression.
23 Megapixel plate images
The image above is an unedited export directly from PhenoBooth+ showing Saccharomyces Cerevisiae colonies at 300% magnification.
Accurate colony picking platform
SI Colony-picking Platform has more than 98% picking accuracy regardless of agar height.
PhenoBooth+ and Stinger
picking accuracy
Enhance your fluorescent lighting options
Screening for fluorescence on agar is advantageous because it is cost-effective and very high-throughput.
While PhenoBooth+ can detect fluorescence, bear in mind this is a macroscopic CCD imaging workstation and not a microscope. As such, it will not detect subcellular or low levels of expression.
Wavelengths
Top and bottom white illumination plus three colour channels included as standard.
Blue, Violet and Cyan
Relative Luminous Intensity vs. Wavelength is shown below. Violet: 50% intensity range from 375 – 400nm, with peak ~390nm. Blue: 50% intensity range from 465 – 475nm, with a peak at 470nm. Cyan: 50% intensity range from 490 – 530nm with a peak of 510nm.
Get in touch
Book a 30-minute slot to discuss your workflow with one of our product experts, or ask us anything.