Streamline analysis
of inhibition assays with PIXL

When screening thousands of isolates, manually measuring zones of inhibition can pose a bottleneck to the discovery of antimicrobial compounds. However, PIXL’s workflow for zone of inhibition analysis enables faster screening without sacrificing accuracy or reliability.

Create a colony picking protocol in minutes

1. Login

Individual user logins, remembered user settings, templates and logs user actions and parameters.

2. Select the plate

Petri or PlusPlate. Pick from the whole plate or define sectors of interest.

3. Detect Zones

Automatically locate central colonies and measure their inhibition zones

4. Refine Zones

Automatically locate colonies of interest for picking.

5. Choose colonies

Filter selected colonies by size, colour, and shape.

6. Select target plate type

Select the number of replicates; on any density, in any pattern, agar, or liquid.

7. Pick colonies

Review your workflow, estimated time, and number of plates required, then press go and walk away.

8. Export data

Review your workflow, estimated time, and number of plates required, then press go and walk away.

Integrate your zone of inhibition analysis with PIXL
and gain a multitude of benefits


Intelligent detection algorithms enable automatic calculation of radii measurements of inhibition zones. By refining selected zones based on size, shape, proximity, and overlap percentage, you can efficiently screen and pick the most promising isolates with antimicrobial potential.

Rapidly isolate and purify
strains of interest

By using PIXL to isolate colonies based on their morphological characteristics, such as size, colour, and shape, it is possible to quickly identify and pick strains from environmental samples for further cultivation and analysis.

Effortlessly pick diverse microorganisms

Out of the box, PIXL is compatible with even the most diverse microorganisms, without the requirement for additional expensive hardware. 2D and 3D mixing of the nozzle head ensures enough colony material is picked for inoculation.


Maintain Sterility

Agar Sensing

Filter Zones

Colony Selection


Which PIXL set-up is right for you?


For those interested in selecting aerobic
microorganisms, a standard version
of PIXL is available.


PIXL, equipped with a light curtain that can be easily integrated with various robotic arm providers..


A customised anaerobic chamber designed for PIXL with user experience in mind, making integration seamless.

Bioprospecting filamentous
actinomycetes with Isomerase

Martin Sim from Isomerase tested PIXL’s ability to pick colonies with atypical phenotypes.

Frequently Asked Questions

90mm Petri dish and OmniTray SBS rectangular plate can be used as source plates for the zone of inhibition workflow. However,
more plate types are supported in other PIXL workflows such as Random colony picking.

Yes, you are able to run the zone of inhibition workflow to image and export rich phenotypic data on each colony and inhibition zone.

The radius is measured in millimetres from the centre of the colony to the edge of the clearing zone.

PIXL’s intelligent algorithm can detect inhibition zones with an overlap of less than 20%.

Yes, PIXL will detect secondary colonies inside the inhibition zones.

Yes, PIXL is able to pick secondary colonies inside the inhibition zone.

Get in touch

Our product experts are on hand to answer your questions about our robots and scopes.
Whether you want to explore your workflow needs, get a quote, request a live demo,
or geek out about science.

What would you like to do?