Fabian Istel, Tobias Schwarzmüller, Michael Tscherner, and Karl Kuchler

Bio Protoc. 2015 Jul 20; 5(14): e1530.

Here, we describe a method enabling the phenotypic profiling of genome-scale deletion collections of fungal mutants to detect phenotypes for various stress conditions. These stress conditions include among many others antifungal drug susceptibility, temperature-induced and osmotic as well as heavy metal or oxidative stress. The protocol was extensively used to phenotype a collection of gene deletion mutants in the human fungal pathogen Candida glabrata (C. glabrata)