Colony Screening Upgrade: PhenoBooth

What is it?

The Colony Screening Upgrade is a software package for the PhenoBooth Colony Imager. Optimised for use with the ROTOR Colony Replication Robot, it offers high-throughput quantitative analysis of your Synthetic Genetic, Yeast 2-Hybrid or Random Mutagenesis arrays. Allowing your image analysis to keep up with your high-throughput pinning.

How does it work?

Use the PhenoBooth to acquire high-resolution images of your colonies on agar plates. Automatically relate control and experimental plates, as well as defining biological and technical replicates. Use inbuilt filters to detect upper and lower thresholds of your phenotype of interest: such as size, circularity, and colour. Normalise for unwanted variables, such as edge effects. Automatically assign gene names to your collection. Then batch apply these filters across all of your images with a single-click. Visualise the hits and export all of your results. Easy!

Why do I need it?

• Find the phenotypes that interest you – Screen colonies by: size, fluorescent intensity, colour, circularity or brightness
• Stop wasting effort – Image Pre-processing, Measurement, Normalisation, Analysis and Visualisation, with a single-click
• Detect hits in real time – Mouse over a colony and see its gene name and variation from control and replicates instantly
• Understand results faster – Inbuilt heatmap visualisations highlight growth differences between your control and experimental conditions
• Get the best results – Nothing compares. Detect every colony, every time, even at 6,144 array density