Genetic analysis of meiotic recombination in Schizosaccharomyces pombe

Smith GR

Methods Mol Biol. 2009;557:65–76

The fission yeast Schizosaccharomyces pombe is well-suited for studying meiotic recombination. Methods are described here for culturing S. pombe and for genetic assays ofintragenic recombination (gene conversion), intergenic recombination (crossing-over), and spore viability. Both random spore and tetrad analyses are described.


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