Fabian Istel, Miha Tome, Sabrina Jenull and Karl Kuchler

Bio-protocol, Vol 7, Iss 07, Apr 05, 2017

Here, we describe a method for a large-scale liquid screening approach in C. glabrata. This liquid media method offers several distinct advantages over solid media approaches. This includes growth measurement on a plate reader instead of comparing growth by eye-sight. Furthermore, the liquid method requires lower amounts of antifungals and offers a higher sensitivity. While this method has been optimized for C. glabrata it might be used for other Candida species and yeast-like fungi as well.