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Large-scale Phenotypic Profiling of Gene Deletion Mutants in Candida glabrata

Fabian Istel, Tobias Schwarzmüller, Michael Tscherner, and Karl Kuchler Bio Protoc. 2015 Jul 20; 5(14): e1530. Here, we describe a method enabling the phenotypic profiling of genome-scale deletion collections of fungal mutants to detect phenotypes for various stress conditions. These stress conditions include among many others antifungal drug susceptibility, temperature-induced and osmotic as well as … Continue reading Large-scale Phenotypic Profiling of Gene Deletion Mutants in Candida glabrata



Phenotypic Profiling of Candida glabrata in Liquid Media

Fabian Istel, Miha Tome, Sabrina Jenull and Karl Kuchler Bio-protocol, Vol 7, Iss 07, Apr 05, 2017 Here, we describe a method for a large-scale liquid screening approach in C. glabrata. This liquid media method offers several distinct advantages over solid media approaches. This includes growth measurement on a plate reader instead of comparing growth … Continue reading Phenotypic Profiling of Candida glabrata in Liquid Media



Systematic Phenotyping of a Large-Scale Candida glabrata Deletion Collection Reveals Novel Antifungal Tolerance Genes

Schwarzmüller T, Ma B, Hiller E, Istel F, Tscherner M, Brunke S, Ames L, Firon A, Green B, Cabral V, Marcet-Houben M, Jacobsen ID, Quintin J, Seider K, Frohner I, Glaser W, Jungwirth H, Bachellier-Bassi S, Chauvel M, Zeidler U, Ferrandon D, Gabaldón T, Hube B, d’Enfert C, Rupp S, Cormack B, Haynes K, Kuchler … Continue reading Systematic Phenotyping of a Large-Scale Candida glabrata Deletion Collection Reveals Novel Antifungal Tolerance Genes