Archive

Making the Connection: Ciliary Adhesion Complexes Anchor Basal Bodies to the Actin Cytoskeleton

Ioanna Antoniades, Panayiota Stylianou, and Paris A. Skourides Developmental Cell 28, 70–80, January 13, 2014 Cilia have been associated with diverse developmental and physiological processes, and defects in cilia underlie a number of genetic conditions. Several lines of evidence support a critical role of the actin cytoskeleton in ciliogenesis and ciliary function. Here, we show … Continue reading Making the Connection: Ciliary Adhesion Complexes Anchor Basal Bodies to the Actin Cytoskeleton



DNAs of simian virus 40 and polyoma direct the synthesis of viral tumor antigens and capsid proteins in Xenopus oocytes

Rungger D, Türler H Proc Natl Acad Sci USA. 1978 Dec;75(12):6073–7 Purified simian virus 40 and polyoma DNAs injected into nuclei of Xenopus oocytes were transcribed and subsequently translated into virus-specifictumor antigens and capsid proteins. Simian virus 40 large and small tumor antigens synthesized in the oocytes were indistinguishable, by gel electrophoresis and [35S]methionine-labeled tryptic … Continue reading DNAs of simian virus 40 and polyoma direct the synthesis of viral tumor antigens and capsid proteins in Xenopus oocytes



Methylation of simian virus 40 Hpa II site affects late, but not early, viral gene expression

Fradin A, Manley JL, Prives CL Proc Natl Acad Sci U S A. 1982 Sep;79(17):5142-6 DNA methylation has been correlated with reduced gene expression in a number of studies, although evidence for a casual link between the two events has been lacking. Because microinjection of simian virus 40 (SV40) DNA into the nucleus of Xenopus … Continue reading Methylation of simian virus 40 Hpa II site affects late, but not early, viral gene expression



Transplantation studies of a putative lymphosarcoma of Xenopus

Asfari M, Thiébaud CH Cancer Res. 1988 Feb 15;48(4):954–7 The fate of the putative transplantable Xenopus lymphosarcoma (M. Balls, Cancer Res., 22: 1142-1154, 1962) was studied under three experimental conditions: (a) xenotransplantation, i.e., transplantation of live “tumor” tissue between adults of Xenopus borealis and X. laevis; (b) inoculation of live “tumor” cells from X. borealis … Continue reading Transplantation studies of a putative lymphosarcoma of Xenopus



Coexpression of glucose transporters and glucokinase in Xenopus oocytes indicates that both glucose transport and phosphorylation determine glucose utilization

Morita H, Yano Y, Niswender KD, May JM, Whitesell RR, Wu L, Printz RL, Granner DK, Magnuson MA, Powers AC J Clin Invest. 1994 Oct;94(4):1373–82 A Xenopus oocyte expression system was used to examine how glucose transporters (GLUT 2 and GLUT 3) and glucokinase (GK) activity affectglucose utilization. Uninjected oocytes and low rates of both … Continue reading Coexpression of glucose transporters and glucokinase in Xenopus oocytes indicates that both glucose transport and phosphorylation determine glucose utilization



Role of the C-terminal tail of the GLUT1 glucose transporter in its expression and function in Xenopus laevis oocytes

Due AD, Qu Z-C, Thomas JM, Buchs A, Powers AC, May JM Biochemistry. ACS Publications; 1995;34(16):5462–71 Structural determinants for the glucose transport kinetics of the erythrocyte glucose transporter have not been established. In this work the role of the cytosolic carboxy-terminal tail in the expression and function of the human GLUT1 isoform in Xenopus oocytes … Continue reading Role of the C-terminal tail of the GLUT1 glucose transporter in its expression and function in Xenopus laevis oocytes



Threonine-for-leucine mutation within domain M2 of the neuronal alpha(7) nicotinic receptor converts 5-hydroxytryptamine from antagonist to agonist

Palma E, Mileo AM, Eusebi F, Miledi R Proc Natl Acad Sci USA. 1996 Oct 1;93(20):11231–5 A study was made of the effects of 5-hydroxytryptamine (5HT) on homomeric neuronal nicotinic receptors (nAcChoR) expressed in Xenopus oocytes after injection of cDNA encoding the wild-type chicken alpha(7) subunit. Acetylcholine (AcCho) elicited large currents (IAcCho) that were reduced … Continue reading Threonine-for-leucine mutation within domain M2 of the neuronal alpha(7) nicotinic receptor converts 5-hydroxytryptamine from antagonist to agonist



Nicotinic agonists competitively antagonize serotonin at mouse 5-HT3 receptors expressed in Xenopus oocytes

Gurley DA, Lanthorn TH Neurosci Lett. 1998 May 15;247(2-3):107-10 The 5-HT3 receptor (5-HT3R) is part of a superfamily of ligand-gated ion channels which includes nicotinic acetylcholine receptors (nAChR). cRNA derived from the long isoform cloned mouse 5-HT3R was used to drive expression of 5-HT3Rs in Xenopus oocytes. 5-HT-induced currents were monitored using two-electrode voltage-clamp. Eight … Continue reading Nicotinic agonists competitively antagonize serotonin at mouse 5-HT3 receptors expressed in Xenopus oocytes



Different functional domains of GLUT2 glucose transporter are required for glucose affinity and substrate specificity

Wu L, Fritz JD, Powers AC Endocrinology. 1998 Oct;139(10):4205–12 GLUT2 is the major glucose transporter in pancreatic beta-cells and hepatocytes. It plays an important role in insulin secretion from beta-cells andglucose metabolism in hepatocytes. To better understand the molecular determinants for GLUT2’s distinctive glucose affinity and its ability to transport fructose, we constructed a series … Continue reading Different functional domains of GLUT2 glucose transporter are required for glucose affinity and substrate specificity