PIXL Pinning Pressure Report

PIXL Pinning Pressure Report

Tom Drake, Singer Instruments, Roadwater, UK.
01/03/2019

Summary

This report covers the measurement and evaluation of the pinning pressure achieved by the PIXL Precision Colony Picker, produced by Singer Instruments.

The pinning pressure was measured using a bespoke test procedure, which replicated the standard pinning functionality of the PIXL. Over the span of 80 separate pinning events the PIXL achieved an average pressure of 6.97 ± 0.74 g/mm2, with a maximum recorded pressure of 8.531 g/mm2.

 
PXL Colony Picker Surface Picking Pressure Report

 

Introduction

When Singer Instruments were asked by their customers to develop a colony picker, one of the main requirements was to outperform the achievable precision and contamination rates of pneumatic firing pins. There are serious concerns that accelerated pin firing damages and/or misses important samples and can contribute to contamination. When designing PIXL, one of the primary considerations was the maximum speed of approach to ensure no chance of microbial material becoming airborne and causing contamination; cell splashing. Meticulous analysis identified the maximum speed of approach to be 19mm/s to ensure no cell splashing, and therefore this is the default approach speed for PIXL.

PIXL uses a novel polymer (PickupLine™) to pick microbial colonies and deposit them on a variety of target plates. The pressure applied to agar plates (and colonies) is vital to ensure agar is not pierced, this guarantees that picking is repeatable and that colonies are not damaged. PIXL was designed to have a gentle picking force and therefore pressure, to never pierce agar. We have minimised the chance of contamination through our regulated speed of approach and pressure. A high speed of approach could induce contamination if cells are forcibly hit by a firing pin causing cell splashing. Regulating contact pressure on every pick is vital to ensure that agar depth can vary across plates and that contamination is minimised whilst protecting microbial samples.

 

Method

To measure the pinning pressure delivered by PIXL, a laboratory scale was used. This was positioned at the source bay location replicating the location of a source plate. The height was representative of an agar filled PlusPlate.

For the testing, a routine was produced that would hold the applied weight to allow a reading to be taken from the scale. Every applied parameter in this routine was set to default to represent a typical picking run. The applied weights were recorded for eighty consecutive pinning events to give a representative sample.
The recorded weights were then converted to pressure, accounting for the diameter of PickupLine as 1mm.

 

Results

The data from eighty consecutive pinning events is presented in Figure 1, with the raw data and calculated pressure shown in Table 1 (below).

 
Figure 1 – Calculated pressure values for all pinning events. All eighty calculated pressure values are shown with their corresponding pin number. An average is shown and suggests there is no increase over time.

 
PIXL Pinning Surface Pressure Figure 1 - Calculated pressure values for all pinning events. All eighty calculated pressure values are shown with their corresponding pin number. An average is shown and suggests there is no increase over time.

 
Each data point recorded is detailed below in Table 1. To account for the diameter of the PickupLine, a surface area of 0.785 mm2 was used to calculate surface pressure.
A mean pressure of 6.97 g/mm2 was calculated, with a standard deviation of ±0.74 g/mm2. The maximum pressure observed was 8.79 g/mm2 and the minimum pressure observed was 5.22 g/mm2.

 
Table 1 – Complete raw weight and calculated pressure for all recorded pinning events. All eighty data points are shown in full below, with corresponding ID, raw weight recorded, and calculated pressure values.

 

ID Weight [g] Pressure [g/mm2] ID Weight [g] Pressure [g/mm2]
1 5.4 6.875 41 6.6 8.403
2 6.9 8.785 42 6.7 8.531
3 5.3 6.748 43 5.7 7.257
4 4.8 6.112 44 5.8 7.385
5 4.2 5.348 45 6.1 7.767
6 4.8 6.112 46 5.5 7.003
7 5.1 6.494 47 5.2 6.621
8 5.0 6.366 48 4.9 6.239
9 5.0 6.366 49 5.2 6.621
10 4.8 6.112 50 5.1 6.494
11 4.9 6.239 51 5.5 7.003
12 5.3 6.748 52 5.6 7.130
13 4.9 6.239 53 5.3 6.748
14 6.1 7.767 54 4.7 5.984
15 6.1 7.767 55 5.2 6.621
16 6.5 8.276 56 5.3 6.748
17 4.1 5.220 57 5.6 7.130
18 5.3 6.748 58 5.2 6.621
19 4.8 6.112 59 4.7 5.984
20 4.6 5.857 60 4.5 5.730
21 5.4 6.875 61 5.9 7.512
22 5.1 6.494 62 5.5 7.003
23 5.1 6.494 63 5.1 6.494
24 5.7 7.257 64 5.4 6.875
25 5.6 7.130 65 5.6 7.130
26 5.7 7.257 66 5.0 6.366
27 5.0 6.366 67 5.3 6.748
28 5.3 6.748 68 5.9 7.512
29 4.9 6.239 69 5.8 7.385
30 5.8 7.385 70 5.6 7.130
31 5.7 7.257 71 6.3 8.021
32 4.7 5.984 72 5.8 7.385
33 5.0 6.366 73 5.8 7.385
34 5.3 6.748 74 6.0 7.639
35 6.2 7.894 75 5.7 7.257
36 6.5 8.276 76 5.8 7.385
37 6.2 7.894 77 5.7 7.257
38 6.1 7.767 78 5.9 7.512
39 6.4 8.149 79 5.5 7.003
40 6.5 8.276 80 5.9 7.512

 

Conclusion

PIXL has demonstrated a consistent picking pressure of under 9g/mm2, with an average pressure of approximately 7g/mm2. This has been attained through regulated contact pressure on every pick, ensuring protection of both the agar surface and microbial sample, whilst minimising the risk of contamination.

 

PIXL Precision Colony Picker

 

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