Replicate Colonies

Transfer arrayed colonies up to densities of 6,144 from one source plate to one target plate or replicate many by pinning one source plate to multiple target plates. Whether that’s agar to agar, agar to liquid, liquid to agar, or liquid to liquid. 

ROTOR+ uses plastic replica plating pads and supports liquid pinning to and from 96 and 384 well microtitre plates and agar pinning at densities 96, 384, 1536, and 6144.

Combine and Break Down Plates

Multiple low-density plates may be combined into fewer high-density plates by offsetting the pins in a square matrix.

Condense your entire 60-plate, 96-well library onto a single plate. Use drastically fewer plates per screen, save >60 fold on reagents, plastic, time, storage space, cost… Likewise, breakdown high-density plates to many low-density plates when required.

See examples of ROTOR+ Pinning Arrays here.

Mate Strains

A protocol to mate strains of yeast quickly from high-density arrays.

Smart Agar-Sensing Pin Head

The pinhead lightly and carefully senses the surface of the agar because it applies a predefined pressure to pick cells up.

Gimbal Mounted Pin Head

The pin head uses a pivoted support that allows the rotation to compensate for fluctuation in agar flatness.

Sterile, Ready-to-use Pin Pads

All Singer PlusPlates and RePads come in gamma-irradiated packs, sterile and ready to use.

UV Light & Anti-Contamination Screen

Protective roller-shutter and UV disinfectant light source for when the robot is not in use and a clear anti-contamination screen during operation.

Super Fast Plate Replication

The robotic print head completes the replication of the entire plate of cells in as little as 25 seconds!

Robotic Lid Lifters

Each lid comes with its own independently-controlled lid lifter, minimising the exposure of cells to open air.

Extremely Easy-to-use Touch-Screen Software

Side-mounted touch-screen computer with simple, user-editable software, Wi-Fi and Ethernet capabilities for online support and software upgrades.

It takes hardly any time to learn how to operate the ROTOR+ and run a program— you can begin running your pinning protocol immediately!

4 Bay Turntable

Four-position colour-coded turntable for loading multiple source or target plates.

3D Liquid Mixing

When spotting liquid to agar, the helical stirring of 96 or 384 well plates is applied by default to maximise cell re-suspension and target plate homogeneity.

2D Dry Mixing

The pinhead can skip around on the agar surface to select from a wider area of cells.

Open API

The ROTOR API is complete and very comprehensive. There is also a detailed help file that includes a getting started guide and full API namespaces.

Kimematic Design for Complete Accuracy & Repeatability

Kinematics are used in the design of the pad hopper, pin head and turntable which ensures that the ROTOR+ knows exactly where the RePads and plates are located. This is how accuracy and repeatability is assured even up to densities of 6,144 and above.

Beer Bottle Opener

It’s been a hard day, time to enjoy a delicious beer—you’ve earned it! (Wine bottle opener also available)