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Construction and high-throughput phenotypic screening of Zymoseptoria tritici over-expression strains

Cairns TC1, Sidhu YS1, Chaudhari YK1, Talbot NJ1, Studholme DJ1, Haynes K2. Fungal Genet Biol. 2015 Jun;79:110-7. doi: 10.1016/j.fgb.2015.04.013. Targeted gene deletion has been instrumental in elucidating many aspects of Zymoseptoria tritici pathogenicity. Gene over-expression is a complementary approach that is amenable to rapid strain construction and high-throughput screening, which has not been exploited to … Continue reading Construction and high-throughput phenotypic screening of Zymoseptoria tritici over-expression strains



The BMP1 gene is essential for pathogenicity in the gray mold fungus Botrytis cinerea

Zheng L, Campbell M, Murphy J, Lam S, Xu JR Mol Plant Microbe Interact. 2000 Jul;13(7):724–32 In Magnaporthe grisea, a well-conserved mitogen-activated protein (MAP) kinase gene, PMK1, is essential for fungal pathogenesis. In this study, we tested whether the same MAP kinase is essential for plant infection in the gray mold fungus Botrytis cinerea, a … Continue reading The BMP1 gene is essential for pathogenicity in the gray mold fungus Botrytis cinerea



Live cell fluorescence imaging for early expression and localization of RIB1 and RIB3 genes in Ashbya gossypii

Sengupta S, Kaufmann A, T S C J Basic Microbiol. 2013 Apr 2;54(1):81–7 Ashbya gossypii is a riboflavin overproducing filamentous fungus. RIB1 and RIB3 genes encode GTP-cyclohydrolase II (GCH II) and DHBP synthase, respectively, the two rate limiting enzymes of the riboflavin biosynthetic pathway. The genes encoding yeast enhanced green fluorescent protein (yEGFP) and mCherry … Continue reading Live cell fluorescence imaging for early expression and localization of RIB1 and RIB3 genes in Ashbya gossypii



Dual mutations reveal interactions between components of oxidative phosphorylation in Kluyveromyces lactis

Clark-Walker GD, Chen XJ Genetics. 2001 Nov;159(3):929–38 Loss of mtDNA or mitochondrial protein synthesis cannot be tolerated by wild-type Kluyveromyces lactis. The mitochondrial function responsible for rho(0)-lethality has been identified by disruption of nuclear genes encoding electron transport and F(0)-ATP synthase components of oxidativephosphorylation. Sporulation of diploid strains heterozygous for disruptions in genes for the … Continue reading Dual mutations reveal interactions between components of oxidative phosphorylation in Kluyveromyces lactis



The F1-ATPase inhibitor Inh1 (IF1) affects suppression of mtDNA loss-lethality in Kluyveromyces lactis

Clark-Walker GD FEMS Yeast Res. 2007 Aug;7(5):665–74 Loss of mtDNA by the petite-negative yeast Kluyveromyces lactis is lethal (rho(o)-lethality). However, mutations in the alpha, beta and gamma subunits of F(1)-ATPase can suppress lethality by increasing intramitochondrial hydrolysis of ATP. Increased hydrolysis of ATP can also occur on inactivation of Inh1, the natural inhibitor of F(1)-ATPase. … Continue reading The F1-ATPase inhibitor Inh1 (IF1) affects suppression of mtDNA loss-lethality in Kluyveromyces lactis