Archive

RNA metabolism is the primary target of formamide in vivo

Rafael Hoyos-Manchado, Félix Reyes-Martín, Charalampos Rallis, Enrique Gamero-Estévez, Pablo Rodríguez-Gómez, Juan Quintero-Blanco, Jürg Bähler, Juan Jiménez, and Víctor A. Tallada Rafael Hoyos-Manchado, Félix Reyes-Martín, Charalampos Rallis, Enrique Gamero-Estévez, Pablo Rodríguez-Gómez, Juan Quintero-Blanco, Jürg Bähler, Juan Jiménez, and Víctor A. Tallada The synthesis, processing and function of coding and non-coding RNA molecules and their interacting proteins … Continue reading RNA metabolism is the primary target of formamide in vivo



Dependency of Heterochromatin Domains on Replication Factors

Leonie Johanna Jahn, Bethany Mason, Peter Brøgger, Tea Toteva, Dennis Kim Nielsen, and Genevieve Thon G3: GENES, GENOMES, GENETICS February 1, 2018 vol. 8 no. 2 477-489; Chromatin structure regulates both genome expression and dynamics in eukaryotes, where large heterochromatic regions are epigenetically silenced through the methylation of histone H3K9, histone deacetylation, and the assembly … Continue reading Dependency of Heterochromatin Domains on Replication Factors



Establishment of expression-state boundaries by Rif1 and Taz1 in fission yeast

Tea Toteva, Bethany Mason, Yutaka Kanoh, Peter Brøgger, Daniel Green, Janne Verhein-Hansen, Hisao Masai, and Geneviève Thona, PNAS January 31, 2017. 114 (5) 1093-1098 In recent years, high-throughput studies have revealed the organization of eukaryotic genomes into chromatin domains, topologically associated domains, and replication domains, laying out important principles for the spatiotemporal organization of the … Continue reading Establishment of expression-state boundaries by Rif1 and Taz1 in fission yeast



Core regulatory components of the PHO pathway are conserved in the methylotrophic yeast Hansenulapolymorpha

Zhou Y, Yuikawa N, Nakatsuka H, Maekawa H, Harashima S, Nakanishi Y, Kaneko Y. Curr Genet. 2016 Jan 21. [Epub ahead of print] To gain better understanding of the diversity and evolution of the gene regulation system in eukaryotes, the phosphate signal transduction (PHO)pathway in non-conventional yeasts has been studied in recent years. Here we … Continue reading Core regulatory components of the PHO pathway are conserved in the methylotrophic yeast Hansenulapolymorpha



Rho1-GEFs Rgf1 and Rgf2 are involved in formation of cell wall and septum, while Rgf3 is involved in cytokinesis in fission yeast

Rho1-GEFs Rgf1 and Rgf2 are involved in formation of cell wall and septum, while Rgf3 is involved in cytokinesis in fission yeast Mutoh T, Nakano K, Mabuchi I The Rho GTPase acts as a binary molecular switch by converting between a GDP-bound inactive and a GTP-bound active conformational state. The guanine nucleotide exchange factors (GEFs) … Continue reading Rho1-GEFs Rgf1 and Rgf2 are involved in formation of cell wall and septum, while Rgf3 is involved in cytokinesis in fission yeast



Molecular analysis of the essential gene for adenylate kinase from the fission yeast Schizosaccharomyces pombe

Konrad M J Biol Chem. 1993 May 25;268(15):11326–34 The enzyme-catalyzed transfer of the terminal phosphoryl group from ATP to an acceptor molecule is an important reaction in a wide variety of biological processes. I demonstrate here the essential function of an ATP:AMP phosphotransferase (adenylate kinase) in the fission yeastSchizosaccharomyces pombe. A cDNA clone encoding immunoreactive … Continue reading Molecular analysis of the essential gene for adenylate kinase from the fission yeast Schizosaccharomyces pombe



Hot spots of recombination in fission yeast: inactivation of the M26 hot spot by deletion of the ade6 promoter and the novel hotspot ura4-aim

Zahn-Zabal M, Lehmann E, Kohli J Genetics. 1995 Jun;140(2):469–78 The M26 mutation in the ade6 gene of Schizosaccharomyces pombe creates a hot spot of meiotic recombination. A single base substitution, theM26 mutation is situated within the open reading frame, near the 5′ end. It has previously been shown that the heptanucleotide sequence 5′ ATGACGT 3′, … Continue reading Hot spots of recombination in fission yeast: inactivation of the M26 hot spot by deletion of the ade6 promoter and the novel hotspot ura4-aim



Cid1, a Fission Yeast Protein Required for S-M Checkpoint Control when DNA Polymerase delta or varepsilon Is Inactivated

Wang SW, Toda T, MacCallum R, Harris AL, Norbury C Mol Cell Biol. 2000 May 1;20(9):3234–44 The S-M checkpoint is an intracellular signaling pathway that ensures that mitosis is not initiated in cells undergoing DNA replication. We identified cid1, a novel fission yeast gene, through its ability when overexpressed to confer specific resistance to a … Continue reading Cid1, a Fission Yeast Protein Required for S-M Checkpoint Control when DNA Polymerase delta or varepsilon Is Inactivated



Involvement of Schizosaccharomyces pombe Srs2 in cellular responses to DNA damage

Wang SW, Goodwin A, Hickson ID, Norbury CJ Nucleic Acids Res. 2001 Jul 15;29(14):2963–72 In the budding yeast Saccharomyces cerevisiae the Srs2/RadH DNA helicase promotes survival after ultraviolet (UV) irradiation, and has been implicated in DNA repair, recombination and checkpoint signalling following DNA damage. A second helicase, Sgs1, is the S.cerevisiae homologue of the human … Continue reading Involvement of Schizosaccharomyces pombe Srs2 in cellular responses to DNA damage